J.of Soc of Agri Struc, Japan.
Vol.28,No.3 December 1997,143-148
Original:
Language: Japanese

    Pollen tube cultivated in vitro is considered to be a simple and useful method qualifying the maturity and quality of composts. However, necessary works to be done previously are to determine the suitable conditions of concentrations of agar, sucrose and boron, as well as pH values, temperatures and culture times for each kind of the pollen used.
    The obtained results of the experiments on the Lycopersion esculentum Mill., Cucurbita moschata Duch, Zea Mays L., Solanum melongena L., and Stewartia pseudo-Camellia Maxim.showed as follows:
1) The suitable concentrations of agar and sucrose for the pollen culture of the Solanum melongena L. and Stewartia pseudo-Camellia Maxim. were 0.8% and 10.0%, respectively.
2) The suitable pH levels were 6.0-6.5 and 6.5-7.0 for the Solanum and the Camellia, respectively.
3) The suitable concentration range of boron for the Solanum was from 8 to 24 ppm, the one for the Camellia was 4 to 16 ppm. Temperature of about 25^oC to 30^oC was enough for both pollen kinds, and the culture times were 16 h. for the Solanum and 8 h. for the Camellia.

Keywords: basic culture medium, culture conditions, pollen tube elongation, Solanum melongena L.and Stewarlia pseudo-Camellia Maxim, compost maturity

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