J.of Soc of Agri Struc, Japan.
Vol.30,No.3 December 1999,247-256
Original:
Language: Japanese

    The fluorescence coenzyme F₄₂₀ was measured as an indicator of methane fermentation. The liquid portion from the methanogenic fermentor was irradiated at an excitation light of 420 nm and observed by fluorescence microscopy. Changes in the fluorescence pixel rate were calculated by an image analyzer during the batch growth of acetotrophic and hydrogenotrophic methanogens. Correlation between the fluorescence pixel rate and the methane production ratio in both methanogens was high. This measurement can potentially help to characterize more metabolic status of the methanogenic bacteria.

Keywords: coenzyme F₄₂₀, fluorescence microscopy, image analysis, methane fermentation, methane production rate

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