Abstract
Major chemical components and properties in whole blood are important indexes for evaluating the nutrition and health conditions of livestock. Therefore, an NlR measuring device called 'BloodSpec-1' using fiber optics in interactance mode was developed to perform the simple and quick analysis of whole blood. A blood collecting tube was used as a sample cell. NIR spectra (700-1050nm) of whole blood samples from goats and from milking cows were measured with the developed device. The calibration and validation for determining oxygen, hemoglobin, and hematocrit were performed by using partial least squares (PLS) regression. As a result, the calibration models with relatively high accuracy could be obtained, indicating that the 'BloodSpec-1' is useful to perform the quick NIR analysis of whole blood. NIR measurement using a blood collecting tube, as a sample cell, seemed to be useful from a practical point of view.HomePageKeywords: NIR spectrum, interactance mode, whole blood, hematocrit, hemoglobin, oxygen goat milking cow