Summary
Two-dimensional electrophoresis of plant protein under starvation of nutrition had been tried to find an index of environmental impact to crops. Three types of tomato nursery plants were sampled. On initial, starvation and recovery stage, plants were cultivated with nutrition until two true leaves expanded fully, then without nutrition for one month and then with nutrition again for two weeks, respectively. The protein spots pattern of each stage was investigated. The spots of three stages showed the same regular pattern and repulsed each other between close distances. The spots counts on the 2-D map location grids were different by the nutrition conditions. On the spots volume distributions of each molecular weight sections, the kurtosis of the starvation stage, which nutrition conditions were very different from the initial and recovery stages, was larger than the initial and recovery stage, which nutrition conditions were same. On the spot volume distributions of each isoelectric point sections, the regression coefficient between initial stage and starvation stage was larger than it between initial stage and recovery stage.HomePageKeywords: two-dimensional electrophoresis, Coomassie Blue staining, spots pattern, kurtosis, regression coefficient